Isoproteins of human apolipoprotein A-I demonstrated in plasma and intestinal organ culture.
نویسندگان
چکیده
منابع مشابه
Hepatic apo-A-I and apo-E and intestinal apo-A-I are synthesized in precursor isoprotein forms by organ cultures of human fetal tissues.
In this report, we demonstrate that organ cultures of human fetal liver and intestine synthesize and secrete into the culture medium the major apolipoproteins. The liver cultures were shown to produce apo-A-I, apo-A11, apo-B, apo-C-11, apo-C-IIIs, and apo-E, and the cultures of intestine produced primarily apo-A-I. The synthesized apolipoproteins secreted into the culture medium were identified...
متن کاملApolipoprotein A4 of primates
Monkey apoA-I was isolated by ultracentrifugation or immunoprecipitation and analyzed by isoelectric focusing and two-dimensional polyacrylamide gel electrophoresis. The plasma apoA-I of 26 Old World monkeys (1 2 cynomolgus and 14 rhesus), 40 New World monkeys (8 cebus, 8 squirrel, 8 spider, 8 owl, and 8 marmosets), 6 prosimians (lemurs) and 10 apes (5 gibbons and 5 chimpanzees) were compared w...
متن کاملBoth apolipoproteins B-48 and B-100 are synthesized and secreted by the human intestine.
Apolipoprotein B (apoB), an apolipoprotein associated with very low density lipoproteins and the atherogenic low density lipoproteins (LDL), directs the metabolism of lipoprotein particles in plasma by interacting with the LDL receptor. Utilizing human intestinal biopsy organ cultures, we have studied the synthesis of intestinal apoB in man. Intestinal organ cultures from normal adults (n = 6) ...
متن کاملlsoproteins of human apolipoprotein A 4 : isolation and characterization
In human serum, polymorphism of apoA-I1 predominantly in HDLs could be demonstrated. HDLS-apoA-I1 was composed of four isoproteins, each with a molecular weight of 8600 (reduced form) and identical immunological properties. The isoproteins are designated apoA-11-1 (PI 5.16), apoA-11-2 (PI 4.89) corresponding to the already known apoAI1 monomer band, apoA-11-3 (PI 4.58), and apoA-11-4 (PI 4.31)....
متن کاملPlasma and hepatic apoE isoproteins of nonhuman primates. Differences in apoE among humans, apes, and New and Old World monkeys.
We have used two-dimensional polyacrylamide gel electrophoresis (PAGE) to study the plasma and hepatic apoE isoproteins of nonhuman primates and have compared them with their human counterparts. We have found that apoE obtained from fresh monkey or ape plasma, as well as nascent apoE synthesized by perfused monkey livers, is composed of several isoproteins that resemble the homozygous (beta) ap...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 255 18 شماره
صفحات -
تاریخ انتشار 1980